Application

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected RANKL in human tonsil tissue sections.

Research CategorySignaling

Anti-RANKL, clone 6A12.1, Cat. No. MABS1696, is a mouse monoclonal antibody that detets RANKL in human cells and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Tumor necrosis factor ligand superfamily member 11 (UniProt: O14788; also known as Osteoclast differentiation factor, ODF, Osteoprotegerin ligand, OPGL, Receptor activator of nuclear factor kappa-B ligand, RANKL, TNF-related activation-induced cytokine, TRANCE, CD254) is encoded by the TNFSF11 (also known as OPGL, RANKL, TRANCE) gene (Gene ID: 8600) in human. RANKL is a single-pass type II membrane protein that is found in high levels in the peripheral lymph nodes and in lower levels in the spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid. Its levels are shown to be up-regulated by T-cell receptor stimulation. RANKL has a cytoplasmic domain (aa 1-47), a helical domain (aa 48-68) and an extracellular domain (aa 69-317). RANKL has been identified to affect the immune system and control bone regeneration and remodeling. RANKL is a cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. It is shown to augment the ability of dendritic cells to stimulate naive T-cell proliferation and is an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. It is shown to induce osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells. RANKL is also reported to activate Akt through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which suggests a role of RANKL in the regulation of cell apoptosis. Three isoforms of RANKL have been described that are produced by alternative splicing. Defects in TNFS11 gene can cause osteoporosis, both a severe autosomal recessive form as well a benign autosomal dominant form.

Immunogen

GST/His-tagged recombinant fragment corresponding to 95 amino acids from the C-terminal region of human RANKL.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Format: Unpurified

Mouse monoclonal antibody in tissue culture supernatant without azide.

Unpurified

Quality

Evaluated by Western Blotting with RANKL recombinant protein.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected human recombinant RANKL protein.

Specificity

Clone 6A12.1 detects RANKL protein in human cells. It targets an epitope within 95 amino acids from the C-terminal region.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~36 kDa observed; 35.48 kDa calculated. Uncharacterized bands may be observed in some lysate(s).